Unfortunately, due to copyright laws, we cannot provide copies of the actual test method. However, the method can be downloaded directly from the ASTM for a relatively low cost. ASTM G ASTM G They may be in the form of films, sheets, molded materials, coatings, foams, tubes, pipes, pucks, tiles, plaques, rods, etc. Other materials, such as paint, drywall, cardboard, fibers, and adhesives may also be tested using the ASTM G21 method.
Test samples are placed on an agar medium that is carbon-free no readily available energy source but contains the basic mineral salts required for fungi to grow. Having a carbon-free medium forces the fungi to grow on the test specimen and not on the surrounding agar surface. After being placed on the mineral salts medium, the test sample is inoculated with a concentrated spore suspension inoculum that contains five different fungal species.
These fungi were chosen by the ASTM based on their affinity for using plasticizers, cellulose, lubricants, stabilizers, and colorants as food sources. The test samples are examined every seven days to monitor progress.
Such films may be prepared by casting on glass and stripping after cure, or by impregnating completely covering filter paper or ignited glass fabric. If the specimen is different on two sides, three specimens of each, face up and face down, shall be tested. Nom 3—In devising a test program intended to reveal quantitative changes occurnng during and after fungal attack, an adequate number of specimens should be evaluated to establish a valid value for the original property.
If five replicate specimens are required to establish a tensile strength of a film material, the same number of specimens shall be removed and tested for each exposure period.
It is to be expected that values of physical properties at various stages of fungal attack will be variable; the values indicating the greatest degradation are the most significant see 4. Reference 2 may be used as a guide. ASTM G21 pdf download. Alternatively, the spore charge can be poured into a sterile glass tissue grinder and gently ground to break up the spore clumps and liberate the spores from the fruiting bodies. Filter the shaken or ground suspension through a thin layer of sterile glass wl in a glass funnel into a sterile flask in order to remove mycelial fragments.
Centrifuge the filtered spore suspension aseptically. Rcsuspcnd the residue in an aliquol of sterile water and centrifuge. If largc mycclia fragmcnts or clumps of agar scrc dislodged during the harvesting, wash the spores in this manner three times to remove possible nutrient carryover from the original cultures. Dilute the final washed residue with sterile nutrient-salts solution see 6. International orders are delivered via courier post services which can be either a postal service, courier service, or a combination of both.
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